The Washing Pool, on the estate at at Great Witley, will be monitored for four years following an outbreak of Koi Herpesvirus
Up to 300 fish have died after contracting a virus in Worcestershire.
The Washing Pool, on the Great Witley Estate, has been closed after an outbreak of Koi Herpesvirus (KHV).
Described as the aquatic equivalent to foot-and-mouth disease, the virus is not harmful to humans but deadly to fish in the carp family.
Government inspectors confirmed the outbreak and controls are now in place to prevent the disease from spreading to surrounding waters.
So far 284 fish, worth a total of £16,000, are known to have died in the pool at Witley Park Farm.
Fred Morris, in charge of fishing pools at the Great Witley Estate, fears the virus could spread to other fisheries
Fred Morris, in charge of fishing pools at the estate, said he had first noticed the problem in early August and notified the Environment Agency.
The fish health inspectorate at the Centre for Environment, Fisheries and Aquaculture Science, external (Cefas) visited and confirmed the outbreak.
Mr Morris said: "We have a big problem in that now we've been notified as a designated area, we come under a four-year oversee by Cefas, which means we can't move fish in our out of the fishery.
"And it gets quite difficult for us because we stop fishing obviously.
"We are concerned fishermen may still come up here and try and fish and that would take the disease to another fishery, which is their biggest concern."
Mr Morris believes KHV was transferred via wet fishermen's nets or a cormorant.
